Biochip Techniques

Chip-based and array-oriented analytics is a major field of activity of the Nano Biophotonics department. Beside the utilization of established biochip techniques the research group develops, tests and establishes novel biomarkers and detection principles for this innovative research field. Small metal nanoparticles are integrated parts in labeling and specific metal enhancement techniques were used for improved signal generation. The nanoparticle biomarkers offer thereby a large potential for signal generation by the detection of bioaffinity interactions at the single molecule as well as the biochip level. Adjustable plasmonic properties of the nanoscale particle labels enable an innovative optical detection of arrays. Additionally, an electrical detection provides a simple and cost-effective alternative for diagnostic purposes. In close collaboration with the Jenaer Biochip Initiative (JBCI) the Nano Biophotonic department aims at novel ways for chip-based bioanalytics, especially at the point-of-care diagnostics.

Optical readout up to single particle level
Array-based electrical readout for point-of-care diagnostics
Protein chip analysis by probing the native fluorescence of proteins
Applications in nano biotechnology, bioanalytics, diagnostics

Optical readout down to single particle level

Nanoparticle marker for optical signal readout by array-based biochips
Sensitivity enhancement down to single particle level by enzymatic or metal-induced silver enhancement

Array-based electrical readout for point-of-care diagnostics

Novel cost-effective readout using resistance measurements

Protein-Chip analysis by probing the native fluorescence of proteins

The aromatic amino acids TRP and TYR fluoresce in the UV range of the spectrum.Their fluorescence lifetime changes upon binding to a ligand (see picture), an aptamer or another protein, which allows for label-free detection of binding events. The protein microarray is analyzed by fluorescence lifetime imaging using time correlated single photon counting.

Applications in nano biotechnology, bioanalytics, diagnostics